Genomics & Transcriptomics

Sample and library preparation, gene sequencing, quantitative PCR studies are carried out for comprehensive molecular studies at the genetic analysis laboratories of İstanbul Microalgae Biotechnologies R&D Center. Within the scope of Project INDEPEDENT, all new generation sequencing applications are successfully implemented with the devices added to the infrastructure.

Information on Services

Small volumes of DNA, RNA and protein concentrations are determined with Implen Nano and Denovix QFX fluorometer devices available in the infrastructure.

The Biorad GelDoc system is a standard gel imaging system, and all nucleic acid and protein-based gel electrophoresis samples are studied.

All quantitative PCR (qPCR) applications are performed in different color channels with a sample capacity of 6 and 384 in the Roche LightCycler 480 real-time PCR system.

The Agilent TapeStation 2000 system is used as a bioanalyzer for quality control of all DNA and RNA samples and sequencing libraries.

The Agilent Bravo NGS system is used in liquid distribution operations where precise low volume and high processing capacity are required, especially in next-generation pre-sequencing sample preparation.

Oxford MinION portable gene sequencing device using Nanopore technology enables in situ gene sequencing applications and contributes to genome sequencing studies with longer gene sequences.

With the Illumina NextSeq 2000 gene sequencing device, genome and transcriptome sequencing, variant analyses, metagenomics and metatranscriptomics studies, methylation sequencing, exome and panel sequencing, amplicon sequencing, ChIP-Seq, ATAC-Seq, acellular sequencing, liquid biopsy analysis, and single-cell sequencing applications can be performed.

Pricing

Please visit our contact page for further information.

Download Device Specifications

Agilent Bravo NGS Automated Liquid Handling Robot
Agilent SureCycler Thermocycler
Agilent Tapestation 4200
GelDoc Go Gel Imaging System
Denovix QFX Florometre
Illumina NextSeq 2000
Oxford Nanopore MinION Portable Gene Sequencing Device
Roche Light Cycler 480 Real-Time PCR